S.K. Maity and N. Roy Chattopadhyay
ABSTRACT
The studies involving regulations of gene expressions need rapid and precise analyses of millions of nucleotide sequences. In silico approaches are fast and accurate, and these save both time and money as these can screen nucleotide sequences to be used in costly molecular biology techniques. Mammalian gene silencing by DNA methylation in 5´CpG3´ dinucleotides play a very crucial role by affecting the remodeling of the underlying chromatin. Therefore the presence of these dinucleotides is studied widely in aberrant expressions of gene(s) seen in abnormal development and in various diseases including cancers. Statistical analyses need exact counts of these nucleotides, which counts may affect the silencing processes. The present report offers a simple in silico approach to determine the counts of these dinucleotides with great precisions and rapid performances involving a countless of nucleotide sequences, without complicated web-based softwares. The modifications of the approach may be used for other sequential patterns also.
Keywords: CpG count, in silico, DNA methylation.