Rajput Rekha, Chandra Amrish
The polyherbal anti-diabetic formulation is prepared in suspension form by mixing the petroleum ether and chloroform extracts of five medicinal plants which are indigenous in India with other excipients because plants are a potential source of anti-diabetic drugs. The anti-diabetic effect of prepared suspension was evaluated at two different doses which were decided by acute toxicity studies by using OECD (Organization for Economic Co-operation and Development) guideline 420.The results of acute toxicity study was revealed that there was no mortality observed at 2000mg/kg for the formulation in mice. Therefore 2000mg/kg dose was considered as cutoff dose so 1/10th and 1/5th of maximum dose was selected i.e.200mg/kg and 400mg/kg, for anti-diabetic studies. It was found that the anti-diabetic activity of formulation in suspension form at a dose of 400mg/kg of body weight (253.33± 0.71) more effective than 200mg/kg of body weight (283.33 ±0.49) on wistar albino rats. It was also showed that the anti-diabetic activity of polyherbal formulation in suspension form is nearly comparable with standard drug glibenclamide (261.5 ±0.56) and the formulation at the dose of 200mg/kg was showed more significant anti-diabetic effect at 1st hour(336.5± 0.76) after single dose drug administration than 400mg/kg body weight but the formulation at dose of 400mg/kg was also showed more significant effect at 4th hours(304.16± 1.01) when its compare with the standard group ( 302.33±0.88) on wistar albino rats and continue this significant effect till 24th hours. So it was concluded that the formulation have significant anti-diabetic activity and also stable till the 3 month which was observed by the stability testing by using different parameters used for evaluation of suspension.
Key words: Polyherbal formulation, Glibenclamide, Suspension