K Supriya, Janardan Chaudhary and S N Shamal


Introduction: Tobacco consumption on prolong may cause cellular damage in liver and lung. This study aimed to observe histopathological changes and oxidative stress on liver and lung in cigarette smoke exposure, nicotine and tobacco induced mice.

Materials: Total 30 male mice were randomly selected, each group contain six mice were as following:  controls (Group I), cigarette smoke exposed (Group II), nicotine induced (group III), Tobacco induced (Group IV) and cigarette smoke exposed plus Tobacco induced (group V) for 45 days each time for 30 minutes. Mice were sacrificed and subjected to histopathological observation and biochemical study for oxidative stress.

Results:  Present study, Histopathological findings of liver showed lymphatic infiltration of hepatocytes, few hepatocytes undergoing mitosis, inflamed kupffer cell. Lung showed lymphatic nodules around bronchus with red cell mass and emphysema with damaged air sacs. Liver and lung show presence of mucus secretion and undergoing fibrosis. MDA level in liver and lung were high (p<0.0001) in Group II 528.85±134 and 331.2±97.61; Group III 667.74±156.92 and 392.6±63.58, Group IV 435.36±101.76 and 300.7±58.69; and Group V 269.76±138.88 and 173.6±66.78 µmol/mg respectively in compared to control group.

Conclusion: Present study concluded that cigarette smoke exposure, nicotine and tobacco induced mice undergo histopathogenicity of lung and liver with increased MDA level.

Keywords: Reactive oxygen species, oxidative damage and histopathogenicity