Priti V. Puppalwar, Prashant Adole, Archana Dhok, Priyanka Bhatkulkar
ABSTRACT
Protection of red cell membranes from free radical-mediated oxidative stress is crucial to the successful management of the sickle cell crisis.
Aims and objectives : To demonstrate in practical terms, the antisickling effectiveness of some micronutrients and vitamins in inhibiting sickle cell hemoglobin polymerization.
Material and methods : The antisickling effectiveness of crude extracts of copper, cobalt, magnesium, two fat soluble vitamins(A,E) and one water soluble vitamin (C) were investigated to ascertain the ability of ions to inhibit sickle cell hemoglobin polymerisation process. Mineral samples of concentration 1.0×10-1 mM, Vitamin A of concentration 100 IU and 1 mg/ml for each of vitamins C and E were prepared and HbSS polymerization experiment was carried out by original methods of Noguchi and Schechter, 1978. Absorbance was took at 700 nm and rates of polymerization were calculated.
Observations and results : Copper (P value-0.253) elevated hemoglobin polymerisation by 33.13% while cobalt( P value-0.635) and magnesium( P value-0.099) inhibited polymerisation by 9.30% and 36.04% respectively. Vitamins A ( P value-0.000), C( P value-0.001) and E ( P value-0.015) inhibited hemoglobin polymerisation by 98.25581%, 62.2093%, 65.69767% respectively.
Conclusion : The results reveal nutritional and therapeutic relevance of antioxidant minerals and vitamins in the management of sickle cell anemia and trait.
Keywords : micronutrients, vitamins, hemoglobin polymerisation, sickle cell disease.