R. Sathiyasundar, K. Valliappan
ABSTRACT
The simple, rapid, sensitive and robust high performance liquid chromatographic method has been developed, optimized and validated for the simultaneous estimation of antiretroviral drugs in pharmaceutical formulation and human plasma samples. First line prescribed nucleoside reverse transcriptase inhibitors used in the treatment against human immunodeficiency virus. The multi-combination therapy has to be proven for efficiently eradicate the HIV, such combinations they are lamividine, stavidine, nevirapine and zidovidine. The method was optimized with the aid of Chemometric tool and the optimized chromatographic condition was obtained Acetonitrile: Methanol: 0.25 % Triethylamine (pH 2.5 adjusted with Ortho phosphoric acid) (20: 20: 60 % v/v/v) at a flow rate of 0.8 ml min-1. The measurement was carried out PDA at 245 nm and the overall run time was less than 10 min and a 4-hydroxy cumarin used as the internal standard (IS). The plasma sample were extracted by simple protein precipitation method and extraction was carried out by using methanol as a co-solvent, then sample were evaporated by air-dry method then reconstituted with mobile phase. The optimized method can be applied for the synchronized quantitative determination of these drugs in formulation and pharmacokinetic, pharmacodynamic investigation of human plasma sample.
Key words: Chemometric, HPLC, zidovidine, lamividine, stavidine and nevirapine